Update 2/27/20. “Do you suffer from sugar cravings, inexplicable fatigue or bloating, anxiety or a “brain fog”? Fortunately, ProBio5 provides a solution to many of today's “mystery” symptoms.” Those are some of the words used to describe the Plexus Slim ProBio5, a healthy gut, probiotic supplement. Interestingly, weight loss is not specifically mentioned in this list, although some may use it for weight loss in conjunction with Plexus Slim (the original pink drink). So, does ProBio5 help weight loss and does it help Plexus Slim work better? Does it have any side effects? Let's see what we can discover. See the review of plexus slim current ingredients, Joyome anti-wrinkle cream and Plexus Hunger Control.
According to the Plexus website, ProBio 5 is supposed to provide these benefits:
- Helps keep intestinal yeast in balance
- Helps support a healthy intestinal tract
- Supports healthy digestion
- Helps improve natural response to imbalance
- Promotes healthy bacteria counts and pH levels in the gut
What Does The Name Mean?
My guess is the “pro” refers to probiotic. The Bio5 in the name refers to the 5 different types of probiotic bacteria in the supplement.
The name “ProBio” gives a hint that this is a probiotic supplement. The “5” in the name tells us that there are 5 different types of probiotic bacteria in the product. Interestingly, there is more in the product than just probiotics. According to the label I have, 1 capsule contains the following ingredients:
|Proprietary blend (200 mg)||% DV NV|
|Chitosinase (from bacillus Sp )|
|Probiotic Blend (100 mg)||NA|
|Vitamin C 150 mg||250 %|
|Grape Seed Extract 25 mg||N/A|
|Vitamin B6 25 mg||50%|
N/A = no daily value established
Other ingredients listed on the label include gelatin, water yeast and brown rice powder.
Note. There is a new ingredients list. See below for a comparison.
Updated ProBio 5 Label
Since this review was created the Nutrition Facts Label of Probio5 has changed. Here is the label as it appears now side-by-side with the original Nutrition Facts labeling:
|ProBio 5 New Nutrition Facts (1 capsule)||ProBio 5 Original Nutrition Facts (1 capsule)|
|Vitamin C 150mg (250%DV)||Proprietary blend (200 mg) consisting of the following:|
|Vitamin B6 2.5mg (125 % DV)||Protease|
|Proprietary Enzyme Blend (200 mg) consisting of the following:||Peptizyme|
|Chitosanase (from Bacillus coagulans)||Chitosinase (from bacillus sp )|
|Cellulase||Probiotic Blend (100 mg) consisting of the following:|
|Seropeptase (as Peptizyme SP)||Lactobacillus|
|Probiotic Blend 100 mg consisting of the following:||Lactobacillus Acidophillus|
|Bacillus coagulans||Bacillus Sporogenes|
|Lactobacillus acidophilus||Bacillus Longum|
|Bifidobacterium longum||S. Boulardi|
|Lactobacillus plantarum||Antioxidant Blend consisting of the following:|
|Saccharomyces boulardii||Vitamin C 150 mg (250% DV)|
|Grape seed extract 25 mg||Grape Seed Extract 25 mg|
|Vitamin B6 25 mg (50% DV)|
As can be seen, some ingredients have remained the same, some have changed and also how some of the ingredients are listed have changed. Note, further below I list another table that compares Probio 5 ingredients. This table is the CURRENT ingredients list. It supersedes the table you see below.
I'll leave up the old table below for those who are doing their own research.
Since the original “pink drink” formula (AKA Plexus Slim. Click this link for my review of plexus slim) had boasted an unpublished study that's been used to substantiate its effectiveness, I wondered if there might be some sort of evidence to support ProBio5? If there is research, I didn’t see any studies about it on the PlexusWorldWide.com.
Likewise, searching for clinical databases for:
No relevant studies showed up.
In addition, when I searched Google for “Probio5 research” no relevant information showed up either. This says to me that ProBio5 ―itself ―probably has no published, peer-reviewed proof that it helps people lose weight or alters bacteria flora.
That said, the idea that certain types of bacteria might help people lose weight (or gain weight) is not without some evidence. Intriguing research appears to show that:
- Overweight mice have different bacteria than skinny mice.
- Bacteria from overweight people transferred to skinny mice, makes mice gain weight.
- Bacteria from skinny people transferred to skinny mice kept mice skinny.
- Bacteria from skinny mice might be less able to breakdown food than bacteria from overweight mice.
- Baby mice, given low doses of antibiotics, have a greater chance of being overweight.
For more info about these types of experiments, see these reviews:
- The microbes in your gut may be making you fat or keeping you thin (WashingtonPost.com)
- How Gut Bacteria Help Make Us Fat and Thin (ScientificAmerican.com)
Just as intriguing is this 2014 study titled Exercise and associated dietary extremes impact on gut microbial diversity which noted that people who exercised intensely (Rugby players) had more of a particular type of bacteria that may play a role in weight loss. These people also had reduced cellular inflammation as well.
For more on this study, see these articles:
- Exercise and the ‘Good’ Bugs in Our Gut (New York Times)
Results such as these, might be the reason for the idea that probiotic supplements can help people lose weight.
But, since I'm not able to find any research on ProBio5 itself, I want to see if there has been any weight loss research on the 5 types of bacteria in the product. If there is, then this might lend evidence to whether it works or not.
Let's do that now.
Proprietary Enzyme Blend
According to the label, there are 4 different types of enzymes in the proprietary enzyme. They are:
This is a protein-digesting enzyme. The prefix “pro” refers to protein and, as a rule, science words that end in “ase” is an enzyme. We need proteases to help us digest proteins.
This is a trademarked name for Serratia Peptidase, another protein-digesting enzyme.
This is an enzyme that breaks down cellulose, a compound that makes up the cell walls of not only microorganism but also of fruits and vegetables. Humans don’t naturally make the cellulase enzyme. As my 12th grade biology teacher once said, many years ago, if we made cellulase, we could digest paper since cellulose is in that too!
This is an enzyme that digests chitin, another type of carbohydrate. In addition to being part of some microorganisms, chitin also forms the outside skeleton of insects and crustaceans.
Ironically, some weight loss supplements contain chitin, such as a product called Chitosan.
According to the Plexus Slim website, they say they did this because fungi and yeast are composed of protein, chitin, and cellulose. The enzymes, they say, help break down these microorganisms, which in turn help friendly, probiotic bacteria flourish.
On the surface, this might make some sense. But, where's the proof? Evidence that these 4 enzymes, reduce yeast or fungal growth – or help people lose weight – could not be located.
In this 2010 article from Newsweek, titled How Intestinal Bacteria May Make You Fat, it's stated that overweight people may have bacteria that work too well―in other words, they are better at digesting food (and thus, absorbing calories) than the bacteria in skinnier people.
If this turns out to be true, would adding enzymes that digest protein and carbs, cause people to breakdown― and absorb― more calories? Would this cause people to gain weight?
I have no idea.
I mention this not to scare anybody but only to give people something to think about. Remember, the weight loss research ― so far that I'm aware of ― does not appear to incorporate protein and carb digesting enzymes.
As such, I don’t think they are needed.
For what it's worth, I personally, I think the enzymes in Probios5 would be destroyed by the acidity of the stomach soon after they are taken. I don’t think the enzymes would make it to the large intestine, where the good and bad bacteria reside.
Now let's look at the bacteria in Probio5. Specifically, I want to search to see if there is any research linking any of these bacteria types to weight loss. If Probio5 works, I think the answer is held with these bacteria.
Probios 5 now contains both Lactobacillus acidophilus and Lactobacillus plantarum.
The first thing we need to know is that there are many types of Lactobacillus bacteria. Plexus Slim does not tell us which type is in Probio5. This is important because it makes reviewing it difficult. With that in mind ―and focusing only on human research ― I searched the national library of medicine for:
- Lactobacillus overweight
- Lactobacillus obesity
- Lactobacillus weight loss
I found the following research that may or may not be relevant:
One animal study noted that Lactobacillus acidophilus injected into animals promoted some weight loss.
Another animal study noted that a specific strain of Lactobacillus plantarum (called “K21” strain) helped weight loss.
Plexus doesn't have this bacteria, but a 2014 study titled Effect of Lactobacillus rhamnosus CGMCC1.3724 supplementation on weight loss and maintenance in obese men and women. In this investigation, overweight men and women were followed for 24 weeks (6 months). When combined with a moderate reduction in calories, the women who used a Lactobacillus rhamnosus supplement lost more weight and fat than women who were given a placebo.
Here is Lactobacillus Rhamnosus on Amazon.
This is a very interesting study but I don’t know if Probio5 contains Lactobacillus rhamnosus or not?
The study also notes that it was only women who lose weight. Men didn’t lose weight.
As was pointed out in this 2014 segment from 60 Minutes, there is a growing consensus that men and women might react differently to some medications. Might this same thing might also be true for supplements?
Focusing on only human studies, I searched clinical databases for these terms:
- Lactobacillus acidophilus obesity
- Lactobacillus acidophilus weight loss
- Lactobacillus acidophilus overweight
Human studies linking Lactobacillus acidophilus to weight loss cannot be located.
Ironically, a review of research it was noted that Lactobacillus acidophilus was associated with gaining weight (not weight loss)!
To be fair, in another study, the researchers concluded that:
“there is no evidence that consumption of lactobacilli or probiotics, in general, would contribute to obesity in humans.”
The lead author of this study is associated with Active Nutrition and Dupont Nutrition and Health, which make probiotic supplements.
I searched the National Library of Medicine for these words:
- Bacillus Sporogenes weight loss
- Bacillus Sporogenes obesity
- Bacillus Sporogenes overweight
No studies showed up for any of these search parameters.
Searching clinical databases for these words:
- Bacillus Longum weight loss
- Bacillus Longum overweight
- Bacillus Longum obesity
revealed no studies of these bacteria and weight loss.
Also called Saccharomyces Boulardii. Saccharomyces boulardii is a type of yeast.
As I reviewed this ingredient, I discovered that the name “Boulardi” is spelled wrong on the ProBio5 label and on Plexus Slim website. I say this because when I searched online for “S Boulardi” all the search results were for “S Boulardii” (there is an extra “i” in the name). Nobody is perfect and I make spelling errors all the time too. I'm sure the company will eventually fix this.
Searching clinical databases for these words:
- Saccharomyces Boulardii weight loss
- Saccharomyces Boulardii overweight
- Saccharomyces Boulardii obesity
In study ,S boulardii was associated with weight loss and reduce fat mass in diabetic mice. Human evidence could not be located.
ProBio 5 Antioxidant Blend
This blend consists of the following ingredients:
- Vitamin C
- Grape seed extract
- Vitamin B6 (pyridoxine)
I can't think of a reason why antioxidants would be needed in a probiotic supplement, except maybe (I'm guessing here…) to help prevent spoilage (and death) of probiotic bacteria?
Grape seeds do contain chemicals (nutrients) called anthocyanins. To be sure, these are healthy things to consume, but some websites report that they inhibit the growth of lactobacillus acidophilus ―one of the main probiotics in ProBio5.
If this is the case, then why is it in this supplement? This is of course, assuming that anthocyanins are the extract used in ProBio5. I'll assume they are not using this extract. Again, what is the extract of grape seeds they are using?
Plexus Slim is also now supposed to help gut health.
ProBio5 New Formula
Before this review was posted, it was discovered that there was a new ProBio5 ingredients list on the Plexus website. Below is the old formula (which I listed above) and the new formula side-by-side so you can see the differences.
|Proprietary Blend (200 mg)||Proprietary Blend (200 mg)|
|Chitosanase (from bacillus coagulans)||Peptizyme|
|Peptizyme SP||Chitosainase (from Bacillus)|
|Probiotic Blend (100 mg)||Probiotic Blend (100 mg)|
|L. Acidophilus||Lactobacillus acidophilus|
|B. Longum||Bacillus Longum|
|L. Plantarum||Bacillus Sporogenes|
|S. Boulardi||S. Boulardi|
|Antioxidant Blend||Antioxidant Blend|
|Vitamin C (150 mg)||Vitamin C (150 mg)|
|Vitamin B6 (2.5 mg)||Grape Seed Extract (25 mg)|
|Grape Seed Extract 25 mg||Vitamin B6 (2.5 mg)|
Red color indicates differences between old and new formulas.
Looking at these labels side by side, the following things stand out to me in the new label:
1. Proteases (plural) replaces Protease (singular).
2. Chitoasanase and Peptizyme have switched positions on the new list. Peptizyme is now called Peptizyme SP.
3. Bacillus Coagulans replaces Lactobacillus. The label also indicates that Bacillus Coagulans contributes both lactobacillus sporogenes and bacillus sporogenes to the product. I searched for weight loss studies for both of these bacteria but could not find any.
4. L. Plantarum replaces Bacillus Sporogenes (why?)
5. There is a LOT less Vitamin B6 – down from 25 mg in the old formula to 2.5 mg in the new product.
6. S. Boulardii is still spelled incorrectly on the new label and website.
Does ProBio 5 Promote Healthy Gut Bacteria?
One of the claims for this supplement is that it's supposed to support a healthy intestinal tract. Does it? Well, there does not appear to be any clinical evidence on the supplement, so this makes knowing whether it does or doesn't, difficult. Let's say it does. The bigger question though is what is a “healthy gut?” What does that look like?
Most experts believe a healthy gut is one that contains a diversity of bacteria. In other words, the greater the number of different types of bacteria in our intestines, the healthier we tend to be.
Can you promote diversity by only eating 5 different types of bacteria?
I don't know.
How To Naturally Promote A Healthy Gut
Most experts on this topic agree that eating foods that contain fiber is a good way to promote a healthy gut. That's because bacteria love to eat fiber – and the more the better.
How Many Live Bacteria?
According to plexusworldwide.com, at the time the product was made, each capsule contains “2 billion probiotic live cells.”
But, this is when the product was made.
This is important and something to keep in mind when shopping for ANY probiotic supplements.
The number of live bacteria present when the supplement was made may not always be the same as what is present when you purchase the product.
How many bacteria are in the product after it's been sitting on a shelf for 6 months old – or 1 year?
ProBio 5 Spit Test
Some people may have heard of a spit test to see if they have high levels of yeast in the body. By spitting in a glass of water, it's said that people have too much yeast if the spit fell to the bottom of the glass, or if it had tendrils hanging below it (like tendrils of a jellyfish).
I've seen the spit test mentioned on several websites including the Dr. Oz website but according to this YouTube video, the spit test ―while quick to do― is not perfect. Several things might make it seem like we have an overgrowth of yeast, when we may not. The best way to know if you have an overgrowth of yeast or fungus is to get tested by your doctor.
Probios 5 Side Effects
The supplement is likely fine in healthy people. No major side effects in healthy people were known when this review was updated.
Here are some general things to consider when taking this supplement. This list is not complete.
- Start with less than recommended for the first week to see if you have any reactions
- Peptizyme (Serrapeptase) might have a blood thinner effect so it might interact with blood thinner medications. This effect is also mentioned on the ProBio5 label.
- Don’t take probiotic supplements if taking antibiotics.
- Stop taking at least 2 weeks before surgery unless your doctor says otherwise
- If pregnant or breastfeeding, talk to your doctor first. This is also mentioned on the ProBio5 label.
- Some research that vitamin C along with grape seed polyphenols might raise blood pressure. The study used more vitamin C than is in ProBio5 – as well as 1000 mg of grape polyphenols (that’s a lot). Since this is only one study its hard to know how relevant it is. talk to your doctor if you have high blood pressure.
- Those with immune system problems should speak to their doctor before taking any probiotic supplement. Weakened immune systems may not be able to handle additional microorganisms.
DoesProBios 5 Work?
Some may take this supplement for weight loss although the proof for its effectiveness could not be located. While some may take the supplement along with Plexus Slim (the pink drink), proof that they work better together can also not be located. Does ProBio5 alter gut health? This is also not known. It might or might not.
What do you think?
Nitza Caraballo says
Thanks for your intensive review of the products. I came across this website because I am trying to get a comparison review of Young Living Life 9 vs Plexus Probio 5.
I’ve been taking Plexus Pro Bio 5, Vitalbiome and Hunger Control for about a month. I suffer from GERD and I was desperately looking for something to help me with all the symptoms. I have also increased my water intake as recommended by my ambassador.
For my surprise, I have been gaining weight instead of actually loosing it. In the other hand, it has helped me to regulate my bowel movements.
In occasions, I get palpitations but I don’t really know if it’s related to the product. In overall, it’s been doing good but I am not totally sold yet.
Ambassadors recommend their products to stop the sugar cravings and mine haven’t stopped. Someone recommended me Young Living Life 9 but I cannot find a decent review on them yet.
Hi Nitza, Let me see what I can dig up about young living life 9. Is that an essential oil product or weight loss supplement? As for plexus, Ive never really thought it could help with sugar cravings but I guess results can vary. Did you see my review of plexus Hunger Control? if not click the link and check it out.
Nitza Caraballo says
Young living is an essential oil company but I guess they have vitamins and other products as well. Thank you!
Nitza, thanks I will take a look at it. It may not be until next week but do stay tuned 🙂
Cody Dowling says
Thank you for the effort on this. It helped me, so I bought from you Amazon affiliate link. Have a great day.
Hi Cody, thanks much and I’m glad it has been helping you 🙂
Just FYI: The amount of Vitamin B6 is exactly the same in the new formulation as it is the old, they are just not listed above side by side. Enjoyed reading your review!
Brenda, thanks for saying that! 🙂
You’ve got some information incorrect above.
L. Plantarum has been added to the formula, but it does NOT replace Bacillus Sporogenes.
Bacillus Sporgenes is also known as Lactobacillus Sporogenes, and both of these are names for Bacillus Coagulans.
Bacillus Coagulans is sometimes referred to as Bacillus Sporgenes because it reproduces by means of spores (it is a “spore-forming” bacteria), while Lactobacillus Sporgenes is considered a misidentification due to the “Lacto” prefix. While it produces lactic acid, it does not digest milk products as do other “Lacto” bacteria. It is now accepted that the more accurate and preferred name for this bacteria is Bacillus Coagulans, rather than either of the two other names that refer to it and many manufacturers of probiotic products have changed their labels to reflect this more accurate and preferred name.
Bacillus Coagulans can be found in a number of probiotic products now (e.g. Ultimate GI Solution, Nexabiotic), and has a number of benefits, including increasing other species of good bacteria, inhibiting the proliferation of bad strains of bacteria, reducing pain, reduction of bloating, reducing symptoms of IBS and Arthritis, reducing intestinal inflamation, “deactivation” of gluten and neutralization of other lectins, enhances protein absorption, and improves muscle recovery, as well as reduces soreness after excercise.
So, Bacillus Coagulans is a very useful strain, as you can see, especially amongst those with IBS or other other symptoms of an inflamed digestive tract, or for athletes who are looking for a solution to the gas that comes from eating large amounts of protein, or for increased results of their athletic endeavors due to its ability to increase protein digestion and absorption, but it was not replaced by L. Plantarum.
Also, you might want to look into the subject of “Biofilms” if you’d like to understand why there are two protein digesting enzymes in the formula. Bad bacteria form a hard protein based coating called a “biofilm” that makes them 1000x harder to affect and to displace with good bacteria. This coating must first be dissolved in order to expose them and remove them from the GI tract. This is why the protein digesting enzymes are included, and if taken on an empty stomach, will pass through the stomach to the small intestine where they will do their work.
One last thing – Saccharomyces boulardii is a type of yeast which has an affinity for the walls of the intestine. It is beneficial because it is a “good” yeast, and once it takes its place along the intestinal wall, bad bacteria cannot attach itself there and repopulate once again. It helps in keeping the 85% to 15% good to bad balance of the intestinal flora that is desirable to have. That’s the purpose and benefit of Saccharomyces boulardii.
GME, thanks for the feedback. it sounds like you know a lot about the probiotics etc in Plexus. Question. Can you tell me the reasons why each probiotic is used? Also you said “B Coagulans is very useful strain” but the name only tells us the genus and species of B coagulans. The label doesn’t tell us the strain of B Coagulans used. Do you know the strain of B Coagulans used -as well as the strains of the other probiotics in plexus slim?
Michele Moore says
Hi, I have a question about the protein-digesting enzymes? If I’m to take this probiotic on an empty stomach in order for them to work, why does the label day with food?
Plus I canNOT take this on an empty stomach or I become nauseated and have heartburn sensations. I’m not arguing with you, I just want to know how best to take this. I’m curious.
Also, being an RN for 30 years now, I believe in probiotics, but the published data is so conflicted.
Hi Michele, in case GME doesn’t reply (he didn’t reply to me), Id say take it with food. That is quite interesting what you said about what distributors say and what the packaging says. When it comes to probiotics, I also like to know what strain of bacteria are used.
Michele, you may also want to consider the fact that Plexus has never disclosed the source of the ProBio ingredients nor have they submitted to a 3rd party quality control. You don’t really know what you’re ingesting when you take a Plexus product. It may not even be a pro-bio ingredient that’s causing the problem but possibly a contaminant.
Wanda Smith says
I have been using plexus slim on and off for quite awhile ,the pink drink after consuming it consistently about a week gives me symptoms of fibromyalgia or arthritis, I do t know if the pro bio 5 does any good or not
Hi Wanda, some may say your symptoms are due to you “detoxing” but I’m not convinced that’s whats going on. I do not know why you are having those symptoms but why dont you try this: stop taking plexus slim and see if the symptoms go away. If they dont, go to your doctor and get checked out. Do let us know what happens.
I do not drink the pinkdrink, but I use the Probio5, MegaX, XFactor multivitamin and the Biocleanse. All my fibromyalgia symptoms have gone away! Been using these supplements since September and have been pain free since October.
You need to contact the 6:00 news on this one. For decades research and billions of dollars has been expended searching for a cure for this horrible debilitating condition. And, bam , all that needed to be done was to throw a bunch of crap without any research into a pot and stir. Amazing!!!!
Thanks so much for this information. When I first started taking Plexus I felt FANTASTIC for about 6 weeks. Loads of energy and I got so much done. But unfortunately it didn’t last. ? Now I’m skeptical. I’d like to see all the research studies they have done. Thanks for all this work. I know it must of taken lots of time to research this.
Hi Mary, thanks for sharing your thoughts about Plexus and for the compliments. Yes, quite a bit of time. My reviews usually are quite in-depth. 🙂
Thank you for the review. I read through a sales pitch on Facebook which said the Plexus system could pretty much heal or help control symptoms of many illnesses. I asked about the research but haven’t gotten a reply yet. In the meantime, it’s nice to read an impartial review.
Hi April, you are very welcome. I’m happy you found the review helpful. Feel free to share it with your friends on facebook
Thank your for this great review. I appreciate your candid and well researched information.
Hi Mike, you are very welcome. Thanks for taking the time to say that 🙂
Thank you for you hard work in this study! I am happy to hear a fellow person who is not an ambassador that is not taken in by the sales gimmicks.
Your comments are well thought out and respectful. I’m cheering for you on the sidelines!