Methylene blue has gained significant attention on social media, with influencers and even some physicians touting it as a powerful supplement for brain health, energy production, anti-aging, and even disease prevention. But how much of this hype is backed by science? Is methylene blue truly a groundbreaking biohack, or are its supposed benefits overstated? More importantly, is it safe for self-administration without medical supervision? This review will also take a critical look at methylene blue through the lens of human clinical trials—examining the scientific evidence behind its effects on cognitive function, mitochondrial health, and potential therapeutic applications. We’ll also explore possible side effects, drug interactions, and safety concerns that are often overlooked in social media discussions. This unbiased analysis will help you make an informed decision about whether methylene blue is worth considering—or if the risks outweigh the potential rewards.
What is Methylene Blue?
Methylene blue (MB), also known as methylthioninium chloride, is a synthetic dye with medical and scientific uses. It has been used to treat methemoglobinemia, a condition where blood can’t carry oxygen properly, and as a staining agent in medical tests to highlight cells and tissues.
In recent years, methylene blue has gained popularity as a nootropic and anti-aging supplement, largely fueled by social media claims about its potential benefits for mitochondrial function, cognitive health, and longevity.
Oxidized vs. Reduced Methylene Blue: What’s the Difference?
Methylene blue can switch between two different forms in the body: oxidized methylene blue (MB⁺) and reduced methylene blue (leucomethylene blue or LMB). This change affects how it works inside cells.
- Oxidized Methylene Blue (MB⁺): This is the blue-colored form of methylene blue that has gotten much attention on social media. In this state, it can accept electrons and help with cellular energy production in the mitochondria. Some researchers believe that at low doses, it may help reduce oxidative stress (damage from free radicals). However, at high doses, it may become a pro-oxidant, meaning it could actually increase oxidative stress and cause cell damage instead of preventing it. One way it can do this is by generating hydrogen peroxide.
- Reduced Methylene Blue (Leucomethylene Blue or LMB): In this form, methylene blue appears colorless and can donate electrons, acting as an antioxidant that helps protect cells from damage. This switching ability between oxidized and reduced states is part of what makes methylene blue unique, but it also means that dosage and how it’s used matter a lot.
How Light Activates Methylene Blue
In some medical applications, when the blue dye is exposed to light, particularly in the red and near-infrared (~630-700 nm), it undergoes photoexcitation, meaning it absorbs light energy and enters an excited state.
In the presence of oxygen this generates reactive oxygen species (free radicals). This reaction is at the heart of the dye's antibacterial and antiviral effects, wound healing, and possible anticancer effects. The process of activating the methylene blue is sometimes called photodynamic antimicrobial therapy (PDT).
FDA-Approved Uses For Methylene Blue
The Food and Drug Administration (FDA) has approved methylene blue for a single medical use: treating methemoglobinemia, a condition in which hemoglobin loses its ability to effectively carry oxygen in the blood. Interestingly, intravenous vitamin C has also been shown to help reduce methemoglobin levels. This might explain why some social media influencers advocate combining methylene blue with vitamin C. However, no clinical studies have specifically investigated the effects of taking a cocktail of methylene blue and vitamin C together in an oral supplement form.
Likewise, no direct human proof indicates that taking vitamin C supplements enhances the absorption of the dye or its reputed benefits.
History of Methylene Blue as a Malaria Treatment
The bioactive blue dye holds a unique place in medical history as the first synthetic drug used to treat malaria, dating back to 1891. Its use persisted through World War II and into the 1950s, with a review of 21 clinical trials describing it as “highly effective.” However, one of its most well-known side effects—blue urine—was a common experience among soldiers who received the treatment.
Today, methylene blue is rarely used on its own for malaria due to the rise of drug-resistant malaria strains. Instead, when prescribed, it is typically combined with more potent antimalarial medications to enhance effectiveness and reduce resistance.
While its role in malaria treatment has diminished, it continues to be explored for other potential medical applications, fueling its growing popularity in the alternative health and biohacking communities.
Methylene Blue and Mitochondrial Health: Separating Fact from Hype
Mitochondria are like tiny batteries inside your cells. They help turn food into energy and keep your body working properly. When mitochondria don’t function well, it’s linked to aging, brain diseases, and other health problems. Some researchers believe that methylthioninium chloride (its scientific name) might help protect mitochondria by working around damaged areas in the energy-making process. This could help reduce cell damage caused by free radicals.
Unlike many antioxidants, the dye doesn’t just neutralize free radicals; it may also prevent their formation by stabilizing mitochondrial function. However, there’s a caveat—at higher doses, methylene blue can actually become pro-oxidant, meaning it may contribute to oxidative stress rather than prevent it.
Animal studies show mixed results. Some research suggests it might help mitochondria work better, but not all studies agree. For example, one study found that methylene blue did not stop bone loss in aging mice, raising questions about how well it really works.
The effects of this metabolic modulator on human mitochondria are unclear. What social media influencers fail to mention is that some studies use a purified version of methylene blue, called hydromethylthionine mesylate (HMTM), which is different from the types people buy online or see discussed in videos. Also, the doses used in animal studies (0.5 to 4 mg per kg of body weight) might not translate into being safe or effective for humans.
Another important thing to consider: No research has compared methylene blue to exercise—which is proven to make mitochondria healthier. Until more human studies are conducted, the notion that methylene blue is a must-have supplement for mitochondria is still mostly hype.
Urinary Tract Infections
In one small study of 15 people (14 women and 1 man), researchers found that a combination of methylene blue and methenamine (a drug sold under the names Hiprex and Urex) helped improve urinary tract infections (UTIs). Interestingly, this combination worked just as well as when methenamine was paired with additional medications, but it caused fewer side effects.
However, it’s still unclear whether methylene blue alone can effectively treat UTIs. More research is needed to understand its role and whether it offers any real benefits without being combined with other drugs.
Does Methylene Blue Support Kidney Function?
Some research suggests that this synthetic blue dye may play a role in kidney health, but the evidence is still limited. Scientists have explored its potential benefits based on a few key mechanisms, including acting as an antioxidant and boosting Nrf2 pathway activity, a process that helps protect cells from damage.
Researchers in New Zelaand and the Netherlands undertook a study which looked at septic shock patients who received an intravenous infusion of the blue dye. Researchers observed an increase in creatinine clearance, suggesting an effect on the glomerular filtration rate (GFR)—a key measure of kidney function. However, while creatinine clearance improved, creatinine levels did not return to normal, and overall kidney function was not restored.
There is no scientific proof that methylene blue significantly improves kidney function when consumers use it at home.
Can Methylene Blue Help with Parkinson’s Disease?
Whether this dye's oxidized or reduced forms have the potential for treating Parkinson’s disease is still in its early stages. So far, most of the evidence comes from animal studies, which suggest that it may increase brain-derived neurotrophic factor (BDNF)—a protein that supports brain cell survival and growth. Additionally, methylene blue appears to help dopamine-producing neurons resist damage from neurotoxins, which could have implications for neurodegenerative diseases.
In theory, individuals taking the day may experience a worsening of Parkinson's symptoms if the dye is due to the effects of the dye on monoamine oxidase, an enzyme that breaks down dopamine and other neurotransmitters. The effects may become more pronounced in those who also take antidepressant medications.
While findings from animal research are promising, it’s important to note that no human clinical trials have been conducted to determine whether methylene actually improves Parkinson’s disease symptoms. Until such studies are done, its potential benefits for Parkinson’s remain purely theoretical.
Light-Activated Methylene Blue: Antimicrobial Potential and Limitations
When exposed to red light (630 to 680 nm), methylene blue generates reactive oxygen species (ROS)—highly reactive free radical molecules that can destroy bacteria, viruses, and other pathogens. This photodynamic effect is the basis for its use as an antimicrobial and antiviral agent.
Evidence from Animal Studies
A review of 13 controlled animal studies found that the dye was effective against a wide range of bacteria, fungi, and parasites, including:
- Escherichia coli (E. coli)
- Methicillin-resistant Staphylococcus aureus (MRSA)
- Morganella morganii
- Candida albicans
- Several other pathogens
These findings suggest that the light-activated dye has broad-spectrum antimicrobial properties, though it remains unclear how well these results translate to reductions in human infections.
Effects on Blood Plasma
Because it interacts with blood components, researchers have tested whether light-activated methylene blue affects plasma quality. In a study of 12 healthy individuals, researchers treated their plasma with the dye and light, then reintroduced it into their bodies. The results showed no negative effects on blood clotting, immune response, or overall health, suggesting that the treatment does not impair plasma function.
Potential Benefits for Herpes Simplex Virus
Research on the dye as a treatment for herpes simplex virus (HSV) dates back several decades. A 1973 study found that 18 out of 20 patients (90%) experienced symptom relief after applying light-activated methylene blue to affected areas. Long-term follow-ups 18 to 30 months later revealed that 85% of patients reported fewer outbreaks, with some seeing a 50% reduction in flare-ups.
A 1975 study involving 15 herpes patients showed that the treatment temporarily eradicated symptoms in 73% of people within two to four days. However, 90% of those who improved later experienced relapses, highlighting the short-term nature of the effects.
Limitations and the Need for Medical Guidance
Despite its antimicrobial properties, the light-activated blue dye may be less effective when pathogens are deeply embedded in tissues, making it crucial for individuals to consult a physician before attempting treatment.
Additionally, at least one in vitro study found that combining methylene blue with Nigella sativa oil (black seed oil) enhanced wound healing. However, no human clinical trials have confirmed these effects.
Lack of Evidence for Viral Infections in Humans
The dye has gained a reputation for being a broad-spectrum anti-virtual agent. Indeed, research does indicate that it has potential against several strains of Zika virus and Dengue virus but human trials need to confirm the benefits. Likewise, no clinical trials have been identified to support its effectiveness against influenza, HPV, norovirus, the common cold, or other viral infections.
While the research is intriguing, its real-world benefits remain unproven in large-scale human studies. Until more data is available, claims of its effectiveness against viral infections should be viewed with healthy skepticism.
Methylene Blue and COVID-19: What Does the Research Say?
Some early in vitro studies (lab research using cell cultures) suggest that methylene blue might have potential against SARS-CoV-2, the virus responsible for COVID-19. However, when tested on live animals, the results were far less promising.
Lab Studies: Effective Outside Cells, But Not Inside
In one study, the blue dye was able to kill the virus when it was outside of cells, meaning it showed some antiviral properties in controlled lab conditions. However, once the virus entered cells and began replicating, methylene blue had no effect on stopping the infection. This raises questions about whether it can be an effective antiviral treatment in real-world infections.
Animal Studies: No Protection Against Infection
When mice were given the dye in their drinking water, it failed to prevent or reduce COVID-19 infection. Additionally, treated mice did not live longer than those that didn’t receive the dye, suggesting it provided no survival benefit.
Human Study: Improved Oxygen Levels, Lowered Mortality
A Phase Two clinical trial involving 80 COVID-19 patients tested whether methylene blue could improve outcomes when added to standard treatment protocols. The results showed that patients who received the dye alongside standard care had:
- Better oxygen saturation levels.
- Lower mortality rates (12.5% vs. 22.5%) compared to those who only received standard care.
It's ‘s important to note that the formula given to the patients was actually a cocktail of the blue dye plus vitamin C and N-acetyl cysteine (NAC). It stated this combo was necessary to convert the dye into its antioxidant form, Leukomethylene Blue. However, since both vitamin C and NAC have therapeutic effects, this study can't prove if the dye-by itself -has COVID benefits.
Additionally, it’s important to note that this was a small study, and more research is needed to determine if the dye consistently improves COVID-19 outcomes.
Bottom Line
Although it has been shown to have some antiviral effects in lab settings, real-world results are mixed. It was ineffective in preventing infection in mice, and while it improved oxygen levels in a small human study (when combined with vitamin C and NAC), this doesn’t mean it can cure or prevent COVID-19. More rigorous clinical trials are needed before this antioxidant dye can be considered a reliable treatment for COVID-19 or other viral infections.
Methylene Blue and Depression: A Potential Antidepressant?
The dye has attracted interest as a potential antidepressant due to its effects on brain chemistry and cellular function. Researchers have explored several ways it may influence mood regulation, though much of the evidence remains preliminary.
How Might Methylene Blue Affect Depression?
Scientists believe the dye could impact mental health through several key mechanisms:
- Inhibiting monoamine oxidase (MAO): MAO is an enzyme that breaks down serotonin and norepinephrine, two neurotransmitters that play a key role in mood regulation. By blocking this enzyme, methylene blue may help increase levels of these neurotransmitters in the brain.
- Enhancing mitochondrial function: Some research suggests mitochondrial dysfunction may contribute to depression, and methylene blue has been shown to improve mitochondrial efficiency in animals.
- Reducing nitric oxide synthesis and increasing BDNF: Nitric oxide plays a role in neuroinflammation, which has been linked to mood disorders. Brain-derived neurotrophic factor (BDNF) supports brain plasticity and neuron survival, but whether the dye significantly impacts these pathways still requires further research.
Human Studies on Methylene Blue for Depression
Study 1: Bipolar Disorder and Depression Symptoms
In a two-year study, researchers gave 31 people with bipolar disorder either 15 mg or 300 mg of methylene blue daily. Those receiving 300 mg experienced significant improvements in depression symptoms, though the treatment had no effect on manic episodes. Another problem: of the 31 participants, only 17 completed the study, raising concerns about the reliability of the results.
Study 2: Severe Depression in Women
In another study, 35 women with severe depression took either 15 mg of methylene blue or a placebo daily for three weeks. The results showed that the group receiving the dye had significantly greater improvements in mood compared to the placebo group.
Limitations and Challenges
While these studies suggest methylene blue may have antidepressant effects, several issues make it unclear whether it could be a viable treatment for depression or bipolar disorder:
- Some studies used non-oral administration, making it difficult to compare results.
- Many trials had small numbers of people, limiting their statistical power.
- There is no established optimal dosage, with studies using anywhere from 15 to 300 mg daily.
- A lack of double-blind, placebo-controlled trials (the gold standard) is a major limiting factor in research.
Final Thoughts
While the dye shows promise as a potential antidepressant, particularly in bipolar depression, because of its limited clinical evidence, inconsistent dosing, and small study sizes, it is not considered a first-line treatment for depression or bipolar disorder. More rigorous, large-scale human studies are needed before it can be recommended for mental health.
Methylene Blue and Brain Health: Can It Protect Memory and Cognition?
Methylene blue has sparked interest in its potential neuroprotective effects due to its effect on mitochondria function and energy metabolism. Here's what we know.
At low doses, the dye acts as an electron cycler in the mitochondrial electron transport chain, helping cells produce energy more efficiently while minimizing reactive oxygen species (ROS) that can damage brain cells. This mechanism supports memory and cognitive function by:
- Enhancing cytochrome oxidase activity, a key enzyme involved in brain metabolism.
- Improving oxygen consumption in the brain, which is crucial for mental performance.
- Protecting neurons from oxidative stress, which is a contributor to neurodegenerative diseases.
Because of these potential benefits, methylene blue has gained popularity as a nootropic supplement, particularly among individuals looking for support with Alzheimer’s disease, mild cognitive impairment (MCI), and age-related cognitive decline.
Animal Studies: Potential Neuroprotective Effects
In animal research, methylene blue has shown promising results in models of vascular dementia and early-stage Alzheimer’s disease.
- In a rat study on chronic cerebral hypoperfusion (reduced brain blood flow), rats receiving 4 mg/kg of the dye daily for 30 days had better memory retention and fewer learning deficits. This suggests the dye might protect brain function in conditions linked to poor circulation, such as vascular dementia.
- Other studies have found that low-dose methylene blue (1–4 mg/kg) helps prevent memory loss and supports learning ability, even in animals under oxidative stress or mitochondrial dysfunction.
While these findings are encouraging, animal results don’t always translate to real-world effects in humans. So what about clinical trials in people?
Human Studies on Methylene Blue and Cognitive Function
Study 1: Memory Enhancement in Healthy Adults
In one small study, researchers in Texas evaluated whether methylene blue could improve memory and brain function in healthy adults. They gave 26 men and women a single 280 mg dose of the dye and tested their short-term memory while measuring brain activity using functional MRI (fMRI).
Results showed:
- A 7% improvement in memory retrieval compared to the placebo group.
- Increased brain activity in areas linked to attention and memory.
However, there was a major limitation: only 6 out of 30 participants (20%) were over age 30, meaning this study doesn’t prove methylene blue enhances memory in older adults or those experiencing cognitive decline.
Study 2: Phase 2 Alzheimer’s Disease Trial
A larger clinical trial tested whether the dye could slow cognitive decline in people with mild to moderate Alzheimer’s disease. Researchers enrolled 321 participants, who were randomly assigned to take:
- 69 mg/day, 138 mg/day, or 228 mg/day of methylene blue capsules.
- Placebo (control group).
The study lasted 18 months, with capsules provided by the biopharmaceutical company TauRx Therapeutics.
Key findings:
- Patients with moderate Alzheimer’s who took 138 mg/day saw a 5.42-point improvement on the ADAS-Cog scale, meaning they experienced less cognitive decline than those on placebo.
- These patients also showed less reduction in cerebral blood flow, suggesting improved brain circulation.
The Lucidity Trial
A larger phase 3 study has also been conducted. Nicknamed the Lucidity Trial, it involved 545 with probably Alzheimer's or mild cognitive impairment. The study was supported by TauRx Therapeutics, a biopharmaceutical company and involved their proprietary form of methylene blue called hydromethylthionine mesylate (HMTM), which is not the same as the blue dye being sold online. While the results appear on the Tau website, the study itself may not yet have gone through the peer-review process.
Final Thoughts
While the blue dye shows potential, particularly for memory and cognitive function, the research is still incomplete. Research involving specific medical formulations such as HMTM may not translate into similar effects for standard methylene blue.
Until more research is done, methylene blue should not be considered a first-line treatment for Alzheimer’s disease, dementia, or memory loss.
Methylene Blue and Cancer: A Potential Therapy or Just a Research Tool?
Methylene blue has been studied for its potential role in cancer treatment, particularly through photodynamic therapy (PDT), and has also been used in diagnostic testing to stain cells, making it easier to detect cancerous tissues.
Preclinical Research: Can Methylene Blue Target Cancer Cells?
Animal studies have explored the dye's effects on breast, colon, and skin cancers. Some of these studies suggest that it may selectively target cancer cells while leaving healthy cells unharmed.
However, much of this research involves intravenous (IV) administration, not oral supplementation. When given via IV, methylene blue was often activated by light as part of photodynamic therapy (PDT)—a technique that uses light-sensitive compounds to destroy cancer cells.
Human Research: Methylene Blue for Radiation-Induced Mouth Ulcers
While methylene blue is not a proven cancer treatment, one human study examined its potential for relieving the side effects of radiation therapy.
- Patients with severe mouth ulcers caused by radiation treatment were given a 0.05% methylene blue oral rinse.
- After two days, those using the oral rinse reported significantly less pain compared to individuals receiving conventional therapy.
This suggests methylene blue may help manage radiation-induced side effects, though it does not imply that it can treat or prevent cancer itself.
The Bottom Line: Promising but Not Proven
While preclinical studies hint at methylene blue’s ability to target cancer cells, there is currently not enough high-quality human research to recommend it as a cancer treatment.
Additionally, there is no strong evidence to suggest the dye prevents cancer. More rigorous clinical trials are needed before it can be considered a viable cancer therapy.
Methylene Blue and Exercise Performance: Does It Boost Endurance or Hinder It?
Because methylene blue influences mitochondrial function, some have speculated that it might enhance physical performance by improving energy production and oxygen utilization. However, research in this area is limited.
Animal Study: No Benefit for Cardiovascular Performance
In one study, dogs running on treadmills were given methylene blue to see if it could enhance cardiac output and oxygen consumption during exercise. The results showed no significant improvement compared to dogs that exercised without the dye, suggesting that the substance did not enhance endurance or aerobic capacity.
Could Methylene Blue Actually Reduce Performance?
While some believe the dye could aid exercise performance, it’s possible that higher doses or certain conditions could have the opposite effect.
- Glucose-6-phosphate dehydrogenase (G6PD) deficiency risk: Individuals with G6PD deficiency are at a higher risk of methemoglobinemia, meaning they could be more susceptible to oxygen transport issues if they take methylene blue. In people with this condition, the dye may break down red blood cells causing anemia.
Bottom Line: No Proven Performance Boost
Despite its effects on mitochondrial function, there is no strong evidence that methylene blue improves exercise performance. While WADA, the World Antidoping Agency, does not ban the substance, more human studies are needed before it can be considered a legitimate exercise-enhancing supplement.
Effects of Methylene Blue on Testosterone
Some early research suggests that methylene blue might have a role in hormone regulation, particularly in cases where testosterone levels drop due to chemotherapy.
Animal Study: Potential Protection Against Chemotherapy-Induced Testosterone Decline
In a preliminary study, researchers tested whether methylene blue could reverse testosterone suppression caused by the chemotherapy drug Cisplatin. When rats were given the dye via intraperitoneal injection, their testosterone levels appeared to recover after being lowered by chemotherapy.
What Does This Mean for Humans?
While these findings are interesting, this was an animal study, and methylene blue is not guaranteed to have the same effect in humans.
Bottom Line: No Human Evidence Yet
At this stage, there is no clinical research showing that methylene blue acts like testosterone replacement therapy or can prevent or reverse testosterone suppression in people. More studies are needed before considering it as a potential therapy for chemotherapy-induced hormone imbalances.
Methylene Blue and Aging: Can It Really Slow the Clock?
Thanks to social media hype—including posts from some physicians—many people are now supplementing with methylene blue in the hope of slowing aging and extending lifespan. But does the science support these claims?
The idea that this blue dye might target aging comes from its effects on mitochondrial function and oxidative stress—three key factors involved in aging.
How Might Methylene Blue Affect Aging?
- Boosts ATP production: the dye enhances energy production in mitochondria, the powerhouses of cells.
- Reduces free radical damage: It helps decrease reactive oxygen species (ROS), but at higher doses, it may become a pro-oxidant instead of an antioxidant.
- Increases oxygen consumption: It appears to enhance oxygen use in the brain, which may support cognitive function.
- Protects skin cells: In lab studies, it's been shown to extend the lifespan of fibroblasts, the skin cells responsible for collagen and elasticity.
- Reduces tau protein: Since tau buildup appears to be linked to Alzheimer’s disease, this suggests a potential neuroprotective effect.
- May boost NAD levels: NAD decline is associated with aging, and methylene blue may help increase NAD, though the evidence is still limited.
Animal Studies: Signs of an Anti-Aging Effect?
Several animal experiments have tested whether methylene blue impacts lifespan and age-related decline:
- Female mice treated with the dye appeared to live longer than untreated mice.
- Treated rats showed improvements in memory and cognitive function.
While these findings are intriguing, animal research doesn’t always translate to humans.
Human Research: Can Methylene Blue Reverse Skin Aging?
One of the few human-related studies looked at progeria, a rare genetic disease that causes rapid aging. When the blue dye was applied to skin fibroblasts, it:
- Boosted collagen and elastin production
- Showed benefits in both healthy individuals and those with progeria
The Verdict: Not Enough Human Evidence Yet
While the biological rationale for the dye as an anti-aging compound makes some sense, there is very little direct human evidence showing it slows aging or extends lifespan.
Is Methylene Blue Safe? Potential Risks and Side Effects
Before considering methylene blue supplementation, it’s essential to consult a qualified healthcare provider. Despite the bold claims on social media, there is very little human research on people taking methylene blue orally, such as placing drops directly on the tongue with an eyedropper. Additionally, many social media videos fail to mention potential side effects.
Common Side Effects Reported in Human Studies
- Nausea and diarrhea
- Dizziness and falls
- Painful or uncomfortable urination
- Skin or urine discoloration (blue or blue-green)
- Mild mouth burning (when used as an oral rinse)
Additionally, blue-green urine may interfere with laboratory tests that detect protein levels in urine, which could lead to false test results. Other lab tests such as blood oxygen level and blood sugar may be interfered with. To reduce problems, stop taking the dye at least two weeks before medical testing.
Who Should NOT Use Methylene Blue?
- People with glucose-6-phosphate dehydrogenase (G6PD) deficiency: Methylene blue can trigger dangerous hemolysis (red blood cell destruction) in individuals with this condition.
- Pregnant or breastfeeding individuals: The effects on a developing baby are unknown, so avoiding use during pregnancy and nursing is best.
- Individuals with high blood pressure: Methylene blue can inhibit nitric oxide production, leading to vasoconstriction (narrowing of blood vessels), which can raise blood pressure.
- Those with kidney disease or kidney failure: Methylene blue is primarily processed through the kidneys, making it potentially unsafe for people with kidney issues. The dye may also reduce blood flow to the kidneys.
Serious Risks: Serotonin Syndrome and Prooxidant Effects
- Serotonin Syndrome Risk: Methylene blue can increase serotonin levels, which may be dangerous for individuals taking antidepressants such as SSRIs and MAO inhibitors.
- Combining the dye with supplements, including 5-HTP or St. John’s Wort, may also increase the risk of serotonin toxicity.
- Prooxidant Effects: Although often promoted as an antioxidant, methylene blue can become a prooxidant at high doses, potentially increasing oxidative stress instead of reducing it.
Toxicity Concerns and Lack of a Known Cure
While higher doses are associated with more side effects, the toxic oral dose of the dye is not well established. Some sources warn that there is no known cure for methylene blue toxicity, making unsupervised use particularly risky.
Final Thoughts: Use With Caution
Methylene blue may have potential health benefits, but self-experimentation carries risks, especially without medical supervision. Since human clinical trials on oral use are limited, and toxicity is not fully understood, caution is advised. If you’re considering using methylene blue, speak with your doctor first to determine whether it’s safe for you.
Is Methylene Blue Ready for Widespread Use? The Science Says Not Yet
Despite the glowing endorsements on social media, the scientific research on methylene blue is far from conclusive. While some research suggests it may have potential benefits, the reality is that it’s not ready for mainstream use by the general public. Until larger, well-designed human studies confirm its benefits—and potential risks—it’s best to approach methylene blue with skepticism rather than assuming it’s a miracle supplement.